Third-party tested: HPLC-UV purity and LC-MS identity are reported on the Certificate of Analysis for the lot listed in the Specifications tab (see our COA page).
The batch COA identifies this material as thymosin beta-4 by LC-MS.
Analytical profile: Molecular formula C212H350N56O78S · Molecular weight approx. 4963 g/mol · CAS 77591-33-4 · Purity method HPLC-UV · Identity method LC-MS · Form lyophilized powder.
Structure and sequence
Thymosin beta-4 is a 43-residue peptide of the beta-thymosin family. The material sold as TB-500 10 mg is identified on its batch COA as thymosin beta-4. Its reference sequence, in one-letter code, is:
Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES-OH
The N-terminal serine is acetylated in the reference structure. Reference values are a molecular formula of C212H350N56O78S, a molecular weight of approx. 4963 g/mol, and CAS 77591-33-4. These are reference figures for the compound, not measurements of a particular lot.
Analytical documentation
- Purity (HPLC-UV): the share of the UV-detected peak area that belongs to the main peptide peak. The posted COA (lot TB-20260521-01, Freedom Diagnostics, reported July 8, 2026) reports 99.79%.
- Identity (LC-MS): the measured mass is compared with the expected mass of thymosin beta-4. This confirms what the material is, not how pure it is.
- Net content: where the COA reports it, the measured amount per vial is printed on the certificate.
- A COA reports results for the sample tested. It is not a guarantee for other lots. Match the lot number on your vial to the certificate on our COA page.
Laboratory handling. Supplied as a lyophilized solid in a sealed vial. Handle it under your facility’s standard laboratory procedures and personal protective equipment. Handling is the responsibility of the qualified research facility.
Specifications
- Product
- TB-500 (thymosin beta-4)
- Label
- 10 mg per vial (measured net content for each batch is on the COA)
- Form
- Lyophilized powder
- Identity (per COA, LC-MS)
- Thymosin beta-4
- CAS number
- 77591-33-4
- Molecular formula
- C212H350N56O78S
- Molecular weight
- approx. 4963 g/mol
- Purity
- Reported on each batch COA. The posted COA (lot TB-20260521-01, Freedom Diagnostics, reported July 8, 2026) shows 99.79% by HPLC-UV, with identity confirmed by LC-MS. Verify this COA with the testing lab (opens third-party site)
- Storage
- Store lyophilized material in a cool, dry place, protected from light and moisture; refrigeration (about 4 °C) is recommended. Handling is the responsibility of the qualified research facility.
View the batch Certificate of Analysis on our COA page.
Research FAQ
What is TB-500 10 mg?
A synthetic research peptide identified on its batch COA as thymosin beta-4 (LC-MS), supplied as lyophilized material for in vitro research. For laboratory research use only.
What form is TB-500 10 mg supplied in?
Lyophilized powder, labeled 10 mg per vial. Measured net content for each batch is on the COA.
What is the purity of TB-500 10 mg?
Purity is reported on each batch COA. The posted COA (lot TB-20260521-01, Freedom Diagnostics, reported July 8, 2026) shows 99.79% by HPLC-UV, with identity confirmed by LC-MS.
Does TB-500 10 mg come with a Certificate of Analysis?
Yes. The batch Certificate of Analysis is on our COA page.
How is TB-500 identified on the COA?
The batch COA identifies this material as thymosin beta-4 by LC-MS.
Where can I review the TB-500 10 mg lab report?
The batch Certificate of Analysis is on our COA page.
How should TB-500 10 mg be stored?
Store lyophilized material in a cool, dry place, protected from light and moisture; refrigeration (about 4 °C) is recommended. Handling is the responsibility of the qualified research facility.
Is TB-500 10 mg for research use only?
Yes. It is sold for in vitro laboratory research only and is not for human or animal use.
Who can purchase TB-500 10 mg?
Sold only to qualified researchers and research institutions.
Learn more
- LC-MS Identity Explained: What the Identity Result Confirms
- Research Peptide Glossary
- Peptide Sequence Notation: How Amino Acid Sequences Are Written
- BPC-157, TB-500 and the 20 mg Blend: Specifications Compared
Important notice
For in vitro laboratory research only. Not for human or animal use. Sold only to qualified researchers and research institutions. We may ask you to verify your organization before we ship, and we cancel and refund any order we believe is for personal use.


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